Abstract
Diesel particulate matter (DPM) is known to infiltrate the body and circulate through the bloodstream, adversely affecting human health. However, liver-specific studies assessing DPM exposure and its effects remain limited. Here, we present a protocol for assessing the effect of DPM on the liver in vivo through hydrodynamic tail vein injection in mice. We describe steps for RNA extraction from liver tissue post-DPM administration, followed by reverse-transcription quantitative PCR (RT-qPCR) to observe changes in gene expression levels.
Keywords:
Cell Biology; Metabolism; Model Organisms; Molecular Biology.