Biodiversity of endosymbiont fungi associated with a marine sponge Lamellodysidea herbacea and their potential as antioxidant producers.

This study aims to isolate endosymbiontic fungi from the marine sponge Lamellodysidea herbacea and to explore their antioxidant potential. Marine-derived fungi, with their vast biodiversity, are considered a promising source of novel antioxidants which can replace synthetic ones. Marine sponges have previously reported bioactive properties that could ameliorate oxidative stress, particularly their associated fungi, producing high-frequency bioactive molecules (adaptogenic molecules) in response to stressors. 19 endosymbiont fungi associated with marine sponges were isolated, and their extracts were evaluated for their antioxidant capacities. Extract of an endosymbiont fungus, isolate SPG6, identified as Alternaria destruens, through surface electron microscopy (SEM) and ITS gene sequencing, showed broad range antioxidant activities (EC(50) values) (free radical scavenging 32.54 mg L(-1), Hydroxyl radical scavenging activity < 0.078 g L(-1), total reducing power 0.114 g L(-1), Chelating power 0.262 g L(-1), H(2)O(2) scavenging activity < 0.078 g L(-1), and Superoxide radical scavenging activity > 5.0 g L(-1)). The extract of isolate SPG6 was fractioned and analyzed through GC-MS. Marine sponge-associated endosymbiont fungi are a rich source of antioxidant molecules. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-024-03972-1.