Novel application of CHUANG BI FU biologic protein sponge in promoting peripheral nerve injury repair by regulating vascular regeneration.

BACKGROUND: The search for effective drugs that promote regeneration after peripheral nerve injury (PNI) has received widespread attention, but little attention has been paid to the potential secondary damage to nerves during neurosurgical procedures. CHUANG BI FU Biologic Protein Sponge (CHUANG BI FU) is a composite biological sponge loaded with basic fibroblast growth factor (bFGF) that has been effectively applied to clinical wounds, but has never been used for repairing peripheral nerves. METHODS: This study used a 3.5-mm nerve defect model in mice, and wrap CHUANG BI FU around the nerve defect site, connecting the distal and proximal ends of the damaged nerve without suturing the nerve. The repair function of the nerve by the wound was tested by detecting mouse footprints, electrophysiology, NF200 staining, CD31 staining, and gastrocnemius muscle Masson staining. RESULTS: It was found that CHUANG BI FU can promote axonal elongation, promote neuromuscular reinnervation function, and promote angiogenesis of regenerating nerves, thereby achieving the function of promoting damaged nerve regeneration. And through cellular level research, it was found that CHUANG BI FU may promote vascular regeneration through the PI3 K-AKT pathway. CONCLUSIONS: Using CHUANG BI FU to repair peripheral nerves can promote vascular regeneration through the PI3 K-AKT pathway and it can greatly reduce the technical difficulty and operation time required by surgical operators.