A naturally occurring SNP modulates thermotolerance divergence among grapevines.

With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association study with transcriptomic profiling to identify TTC4 (thermotolerance on chromosome 4), a gene encoding a WRKY transcription factor, as a key determinant of thermotolerance in grapevine. TTC4 directly activates two thermotolerance-related genes, HSP18.1 and APX3. We also identify a heat-suppressed repressor SPL13 (SQUAMOSA-promoter binding protein-like 13) that cannot bind to the GTAT element (TTC4(T(7631))) in intron 2 of TTC4, but can bind to the natural variant, GTAC (TTC4(C(7631))). Grapevine accessions with TTC4(C/C(7631)) genotype exhibit significantly lower thermotolerance compared to those with the TTC4(T/T(7631)) and TTC4(C/T(7631)) genotypes. This fine-tuned regulation contributes to thermotolerance divergence among grapevine populations. The TTC4(T(7631)) haplotype holds significant potential as a genetic resource for breeding thermotolerant grapevine varieties.