The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model.
利用绒毛尿囊膜模型进行 BxPC-3 胰腺肿瘤异种移植的方案
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作者:Colenbier Robin, Coppens Lenny, Logghe Tine, van Zwol Eke, Timmermans Jean-Pierre, Bogers Johannes
| 期刊: | STAR Protocols | 影响因子: | 1.300 |
| 时间: | 2024 | 起止号: | 2024 Jun 21; 5(2):102968 |
| doi: | 10.1016/j.xpro.2024.102968 | 研究方向: | 肿瘤 |
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