Cashew nut oligopeptide inhibits mast cell degranulation by suppressing activation of the NF-Κb signalling pathway.

This study aimed to isolate oligopeptides with anti-allergic properties from cashew nuts. Cashew nut protein was extracted using subcritical water extraction. Subsequently, cashew nut oligopeptides (COPs) were obtained via a two-step enzymatic hydrolysis involving alkaline proteinase and bromelain. Mass spectrometry analysis identified a specific COP with the sequence Ile-Ile-Ala-Ile-Pro-Ala-Gly-Val-Ala-His. This COP was synthesised to investigate its impact on preventing degranulation. Analysis revealed that COP effectively inhibited the release and production of proinflammatory mediators, including tumour necrosis factor-α, interleukin-6, histamine and β-hexosaminidase and influx of extracellular calcium ions. Furthermore, COP significantly suppressed degranulation and inflammatory responses by inhibiting activation of nuclear factor kappa B signalling pathway in allergic mast cells. These findings suggest that COP possesses strong anti-allergic effects by specifically targeting mast cells degranulation. This s highlights COP's therapeutic potential against allergic inflammatory disorders, and its promising role in treating allergic diseases.