Potato protein hydrolysate (PPH902) exerts anti-lipogenesis and lipolysis-promoting effect by inhibiting adipogenesis in 3T3-L1 adipocytes.

Obesity is linked to cardiovascular disease, cerebrovascular disease, diabetes, and dyslipidemia, lowering quality of life, work productivity, and healthcare expenditures. The aim of this present study is to investigate the mechanism of potato protein (PP) post-treatment in regulating lipogenesis and lipolysis in 3T3-L1 adipocytes. 9% PP hydrolysed for 2 h (PPH902) shows high yield and better activity; thus, PPH902 was used in all other experiments. 3T3-L1 preadipocyte cells were used, the cell culture medium were changed every 2 days, IDH was added on Day 0. PPH902 was added on the 8th day, it was left for 72 h and then cells are collected. The relative triglyceride residual (RTR) content was quantified, and the expression of key lipid metabolism-related proteins was analyzed using Western blotting. PPH902 at concentrations of 400 ppm, 800 ppm, and 1600 ppm markedly decreases the RTR content. PPH902, at higher doses, modulates the expression of lipid production-associated transcription factors PPARγ, SREBP-1c, and FAS by activating AMPK, which inhibits lipogenesis and activates phosphorylated HSL to enhance lipolysis, so augmenting lipid metabolism. These findings suggest that PPH902 is an effective anti-lipogenic and lipolysis-promoting agent with potential applications in anti-obesity interventions.