Silver Nanoparticles at Low Concentrations Embedded in ECM Promote Endothelial Monolayer Formation and Cell Migration.

Several scientific studies have reported the opposing effects of silver nanoparticles (AgNPs) on angiogenesis, ranging from proangiogenic to anti-angiogenic. The widespread use of AgNPs in biomedical applications and the variability of their effects depending on concentration and exposure conditions highlight the need for further research into their impact on vascularization and endothelial cell behavior. This study aimed to investigate the potential influence of AgNPs on human umbilical vein endothelial cells (HUVECs) using a model incorporating a thin layer of an extracellular matrix (ECM). To this end, cytotoxicity was assessed, and endogenous nitric oxide and superoxide levels were measured. Additionally, the effects of AgNPs on HUVEC confluence and migration were evaluated. The expression levels of 43 proteins involved in angiogenesis were also analyzed. The results revealed that ECM enriched with AgNPs at a concentration of 0.5 mg/L enhanced cell coverage, promoted migration, and supported monolayer formation without inducing cytotoxicity.