Dietary glycine supplementation enhances glutathione availability in tissues of pigs with intrauterine growth restriction.

This study tested the hypothesis that dietary supplementation with glycine enhances the synthesis and concentrations of glutathione (GSH, a major antioxidant) in tissues of pigs with intrauterine growth restriction (IUGR). At weaning (21 d of age), IUGR pigs and litter mates with normal birth weights (NBW) were assigned randomly to one of two groups, representing supplementation with 1% glycine or 1.19% l-alanine (isonitrogenous control) to a corn- and soybean meal-based diet. Blood and other tissues were obtained from the pigs within 1 wk after the feeding trial ended at 188 d of age to determine GSH, oxidized GSH (GSSG), and activities of GSH-metabolic enzymes. Results indicated that concentrations of GSH†+†GSSG or GSH in plasma, liver, and jejunum (P†<†0.001) and concentrations of GSH in longissimus lumborum and gastrocnemius muscles (P†<†0.05) were lower in IUGR pigs than in NBW pigs. In contrast, IUGR increased GSSG/GSH ratios (an indicator of oxidative stress) in plasma (P†<†0.001), jejunum (P†<†0.001), both muscles (P†<†0.05), and pancreas (P†=†0.001), while decreasing activities of γ-glutamylcysteine synthetase and GSH synthetase in liver (P†<†0.001) and jejunum (P†<†0.01); and GSH reductase in jejunum (P†<†0.01), longissimus lumborum muscle (P†<†0.01), gastrocnemius muscle (P†<†0.05), and pancreas (P†<†0.01). In addition, IUGR pigs had greater (P†<†0.001) concentrations of thiobarbituric acid reactive substances (TBARS; an indicator of lipid peroxidation) in plasma, jejunum, muscles, and pancreas than NBW pigs. Compared with isonitrogenous controls, dietary glycine supplementation increased concentrations of GSH plus GSSG and GSH in plasma (P†<†0.01), liver (P†<†0.001), jejunum (P†<†0.001), longissimus lumborum muscle (P†=†0.001), and gastrocnemius muscle (P†<†0.05); activities of GSH-synthetic enzymes in liver (P†<†0.01) and jejunum (P†<†0.05), while reducing GSSG/GSH ratios in plasma (P†<†0.001), jejunum (P†<†0.001), longissimus lumborum muscle (P†<†0.001), gastrocnemius muscle (P†=†0.01), pancreas (P†<†0.05), and kidneys (P†<†0.01). Concentrations of GSH plus GSSG, GSH, and GSSG/GSH ratios in kidneys were not affected (P†>†0.05) by IUGR. Furthermore, glycine supplementation reduced (P†<†0.001) TBARS concentrations in plasma, jejunum, muscles, and pancreas. Collectively, IUGR reduced GSH availability and induced oxidative stress in pig tissues, and these abnormalities were prevented by dietary glycine supplementation in a tissue-specific manner.