In Vivo, Evaluation of Wound Healing Activity of Nanoliposomes Loaded Withania somnifera Extract.

PURPOSE: Medicinal plants and their derivatives have been used to treat wounds, and loading the plants into nanoliposomes (NLPs) helps to increase their efficacy. This study investigated the efficacy of NLPs loaded with Withania somnifera (WHSE) extract in mouse models for excisional wound healing. METHODS: In the present study, we thoroughly evaluated WHSE's antibacterial, antioxidant, and safety profiles. Additionally, we assessed wound contraction, pathological evaluations, and the expression of basic fibroblast growth factor (bFGF) and CD31. RESULTS: The results showed that the extract and its NLPs had biocompatibility and exhibited antibacterial and antioxidant properties. Furthermore, our in vivo wound healing assay results showed that ointments containing 0.50% and 1.00% of the WHSE-NLPs accelerated wound healing and increased collagen and epithelialization. Furthermore, the results of the immunofluorescence and immunochemical tests indicated more expression of CD31 and bFGF in the mice that have been treated with WHSE-NLPs compared to those who were treated with WHSE and control groups. (P<0.05). CONCLUSION: We demonstrated that the administration of 1.00% of the WHSE-NLPs could compete with the commercial ointment (Nitrofurazone®). Therefore, balms prepared from WHSE-NLPs expedited the wound healing process by increasing collagen, epithelialization, and the expression of CD31 and bFGF.