Protocol for the quantitative characterization of cell aggregates using an MRI setup maintaining optimal cultivation conditions.

The use of destructive biochemical assays and the preparation of histologic samples are routinely employed to monitor development and viability of 3D cell aggregates. Magnetic resonance imaging (MRI) offers a non-destructive, high-resolution alternative to histological analysis, enabling longitudinal assessment of cellular dynamics while preserving sample integrity. Here, we present a protocol for non-invasive MR imaging of cell spheroid clusters by creating an adequate imaging environment. We describe steps for spheroid formation, casting of the imaging tube, cell cultivation, and data evaluation. For complete details on the use and execution of this protocol, please refer to Wißmann et al.(1).