T-PRP-DAT Gel: A Novel Material Promotes Adipose Tissue Regeneration.

BACKGROUND: Decellularized adipose tissue (DAT) has emerged as a promising tissue-specific regenerative platform for soft tissue augmentation and reconstruction. Hydrogels are a widely used DAT scaffold format for their injectability and porous structure. While unstable structure and poor vascularization limit the adipose tissue regeneration of DAT gels, this yields significant clinical necessity for solutions to this problem. METHODS: Based on collagen-fibrin interpenetrating, we developed an injectable thermosensitive DAT/PRP interpenetrating polymer network (t-DP gel/t-DPI) with the composition of DAT and temperature-controlled platelet-rich plasma (t-PRP). The same volume of t-DP gel and DAT gel were transplanted in a mouse model, and graft volume, weight, adipose tissue regeneration rate, and vascularization were compared. RESULTS: The t-DPI showed reinforced stability for the interpenetrating polymer network (IPN) of collagen-fibrin and sustained release of growth factors from the t-PRP, resulting in improved graft volume, weight, adipose tissue regeneration, and vascularization. CONCLUSIONS: Compared with traditional DAT gel, t-DP gel promotes adipose tissue regeneration by promoting angiogenesis and stability, and t-DP gel has great potential for future applications in the field of plastic surgery for its simple preparation and regeneration ability.