Comprehensive Evaluation of the Safety and Efficacy of MOSY PHYCOLLREPAIR: A Novel Fermented Black Bee Honey-Derived Cosmetic Ingredient.

BACKGROUND: MOSY PHYCOLLREPAIR is an innovative cosmetic ingredient extracted from fermented blank bee honey, specifically engineered for dermatological applications. OBJECTIVE: This study seeks to thoroughly evaluate the safety and diverse efficacy profiles of MOSY PHYCOLLREPAIR within cosmetic formulations. METHODS: The safety of MOSY PHYCOLLREPAIR was determined through zebrafish embryo developmental toxicity assays. Its dermatological benefits were assessed via a comprehensive series of in vivo zebrafish embryo tests, encompassing antioxidation, anti-inflammation, modulation of type I collagen, elastin, and sirt1 gene expression, wound healing, skin barrier integrity, UV protection, and skin whitening. RESULTS: MOSY PHYCOLLREPAIR demonstrated non-toxicity to zebrafish embryos at concentrations up to 3.08 mg/mL. It showed significant efficacy across multiple parameters: 29.10%-62.48% anti-ROS activity, 11.08%-27.84% anti-inflammatory effect, 60.25%-295.67% ATP-enhancing efficacy, and 6.52%-17.80% melanin inhibition at concentrations ranging from 25 to 500 μg/mL. It also upregulated the expression of type I collagen genes col1a1 by 87.67% to 281.61%, col1a2 by 54.08% to 165.14%, astin gene elna by 55.81% to 174.03%, and sirt1 gene by 60.25%-295.67%. Additionally, MOSY PHYCOLLREPAIR enhanced wound healing by 8.33%-19.44% at concentrations between 5 and 100 μg/mL, provided 39.36% to 70.26% skin barrier protection against chemical peel at 100 to 500 μg/mL, and offered 20.15%-68.45% UVB irradiation protection at 200 to 2000 μg/mL. CONCLUSION: This study marks the first application of fermented blank bee honey as an active cosmetic ingredient. The findings conclusively establish MOSY PHYCOLLREPAIR as a safe and highly effective cosmetic component, delivering a broad range of anti-aging and skin health benefits.