Mosquito Species Diversity and Circulation of Mosquito-Borne Viruses in Selected Provinces of Central Vietnam.

Arboviruses, including but not limited to dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV), pose a significant global threat to human health. The transmission of DENV, ZIKV, and CHIKV is facilitated by mosquitoes belonging to the genus Aedes, which are prevalent in both urban and rural regions of Vietnam. In 2023 an investigation into the population of mosquitoes was conducted in a number of provinces located within the central region of Vietnam. A total of 12,546 mosquitoes were collected during the study. The mosquitoes collected comprised the genera Culex spp., Aedes spp., Anopheles spp., and Armigeres spp. The Aedes spp. mosquitoes were predominant, being collected in 908 pools. These were then examined by RT-qPCR for the detection of DENV, ZIKV, and CHIKV. DENV viral RNA was detected in 92 mosquito pools, ZIKV was detected in 1 mosquito pool, and CHIKV was not detected. The typing of samples containing DENV RNA was carried out. It is evident from the results of the typing process that three distinct types of DENV have been identified. The three main dengue virus types are DENV-1, DENV-2, and DENV-4.