Use of the omp50 gene for identification of Campylobacter species by PCR

利用 omp50 基因通过 PCR 鉴定弯曲杆菌种

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作者:Luc Dedieu, Jean-Marie Pagès, Jean-Michel Bolla

Abstract

We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.

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