A transgene design for enhancing oil content in Arabidopsis and Camelina seeds

提高拟南芥和亚麻荠种子含油量的转基因设计

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作者:Yerong Zhu #, Linan Xie #, Grace Q Chen, Mi Yeon Lee, Dominique Loque, Henrik Vibe Scheller

Background

Increasing the oil yield is a major

Conclusion

The findings suggested that the maize master regulator, ZmLEC1, driven by a downstream seed-specific promoter, can be used to increase oil production in Arabidopsis and Camelina and might be a promising target for increasing oil yield in oilseed crops.0.

Results

Agrobacterium-mediated transformation successfully generated Arabidopsis and Camelina lines that overexpressed ZmLEC1 under the control of a seed-specific promoter. This overexpression does not appear to be detrimental to seed vigor under laboratory conditions and did not cause observable abnormal growth phenotypes throughout the life cycle of the plants. Overexpression of ZmLEC1 increased the oil content in mature seeds by more than 20% in Arabidopsis and 26% in Camelina.

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