Elevated urinary urea by high-protein diet could be one of the inducements of bladder disorders

Previous work found that urea accumulation in urothelial cells caused by urea transporter B knockout led to DNA damage and apoptosis that contributed to the carcinogenesis. The

We provided evidences that high urinary urea concentration caused by high-protein diet might be a potential carcinogenic factor in bladder.

A high protein diet rat model was conducted by feeding with 40 % protein diet. In-silico modeling and algorithm, based on the

Based on the analysis of different expressed mRNAs/proteins, 15 significant ones (CRP, MCPT2, MCPT9, EPXH2, SERPING1, SRGN, CDKN1C, CDK6, CCNB1, PCNA, BAX, MAGEB16, SERPINE1, HSPA2, FOS) were highly identified and verified by qPCR and immunohistochemistry. They were involved in immune and inflammatory response, cell cycle arrest, apoptosis and pathways in cancer. These abnormally activated processes caused the bladder interstitial congestion and inflammatory infiltrates under the thinner urothelium, cell desquamation, cytoplasm vacuolization, nucleus swelling and malformation in the high-protein diet group. Conclusions: We provided evidences that high urinary urea concentration caused by high-protein diet might be a potential carcinogenic factor in bladder.