Modified multiplex PCR method for detection of pyrogenic exotoxin genes in staphylococcal isolates

改良多重 PCR 方法检测葡萄球菌分离株致热外毒素基因

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作者:Astrid Løvseth, Semir Loncarevic, Knut G Berdal

Abstract

A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.

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