Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR
温度、介质和保存时间对 PCR 检测沙眼衣原体 DNA 的影响
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作者：Laura van Dommelen, Petra F G Wolffs, Frank H van Tiel, Nicole Dukers, Selma B Herngreen, Cathrien A Bruggeman, Christian J P A Hoebe
Abstract
We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.
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