Abstract
This article has been retracted: please see Elsevier policy on Article Withdrawal (https://www.elsevier.com/about/policies-and-standards/article-withdrawal). This article has been retracted at the request of the authors. In Liang et al., the authors recently identified that the CRISPR library used in the pooled screens targeting long noncoding RNAs (lncRNAs) and protein-coding transcripts was not filtered to remove potential off-targets elsewhere in the human transcriptome (as described in the “Method details”). The authors are grateful to the lab of Dr. Roland Rad for bringing this to their attention and apologize for this error. After proper filtering for potential off-targets by removing CRISPR guide RNAs (gRNAs) with up to 2 mismatches to protein-coding transcripts or lncRNAs, the authors have re-analyzed the pooled CRISPR screens. Also, they have updated experiments involving lncRNAs that were targeted using gRNAs with potential off-targets. The updated analysis and experiments are available as a preprint (http://doi.org/10.6084/m9.figshare.30370171). Given the changes in essential lncRNAs identified after proper gRNA filtering, the authors wish to retract the paper and apologize for any inconvenience this may have caused. While the authors work to publish the corrected study, they hope that the updated preprint will be helpful and encourage the community to reach out with any questions.
Keywords:
CRISPR-Cas13; MALAT1; MIR17HG; RNA targeting; SLC16A1-AS1; essential genes; human development; lncRNA; noncoding RNA; transcriptome scale; tumor biomarkers.