Chlorine Modulates Photosynthetic Efficiency, Chlorophyll Fluorescence in Tomato Leaves, and Carbohydrate Allocation in Developing Fruits.

Chlorine (Cl) is an essential nutrient for higher plants, playing a critical role in their growth and development. However, excessive Cl application can be detrimental, leading to its limited use in controlled-environment agriculture. Recently, Cl has been recognized as a beneficial macronutrient, yet studies investigating its impact on plant growth and fruit quality remain scarce. In this study, we determined the optimal Cl concentration in nutrient solutions through a series of cultivation experiments. A comparative analysis of Cl treatments at 1, 2, and 3 mM revealed that 3 mM Cl(-) significantly enhanced chlorophyll content, biomass accumulation, and yield. Furthermore, we examined the effects of 3 mM Cl(-) (supplied as 1.5 mM CaCl(2) and 3 mM KCl) on leaf photosynthesis, chlorophyll fluorescence, and fruit sugar metabolism. The results demonstrated that Cl(-) treatments enhanced the activity of Photosystem I (PS I) and Photosystem II (PS II), leading to a 25.53% and 28.37% increase in the net photosynthetic rate, respectively. Additionally, Cl(-) application resulted in a 12.3% to 16.5% increase in soluble sugar content in mature tomato fruits. During fruit development, Cl(-) treatments promoted the accumulation of glucose, fructose, and sucrose, thereby enhancing fruit sweetness and overall quality. The observed increase in glucose and fructose levels was attributed to the stimulation of invertase activity. Specifically, acidic invertase (AI) activity increased by 61.6% and 62.6% at the green ripening stage, while neutral invertase (NI) activity was elevated by 56.2% and 32.8% in the CaCl(2) and KCl treatments, respectively, at fruit maturity. Furthermore, sucrose synthase (SS-I) activity was significantly upregulated by 1.5- and 1.4-fold at fruit maturity, while sucrose phosphate synthase (SPS) activity increased by 76.4% to 77.8% during the green ripening stage. These findings provide novel insights into the role of Cl(-) in tomato growth and metabolism, offering potential strategies for optimizing fertilization practices in protected horticulture.