Protein Expression Protocol for the Catalytic Domain of an Adenylate Cyclase Membrane-Anchored by a Vibrio Quorum Sensing Receptor

弧菌群体感应受体锚定的腺苷酸环化酶膜催化结构域的蛋白质表达方案

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作者:Stephanie Beltz, Joachim E Schultz

Abstract

The direct regulation of a mycobacterial adenylate cyclase (Rv1625c) via exchange of its membrane anchor by the quorum sensing receptor CqsS (Vibrio harveyi) has recently been reported ( Beltz et al., 2016 ). This protocol describes the expression and membrane preparation for these chimeric proteins.

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