The Antioxidant, Antimicrobial, and Antitumor Proprieties of Flavonol-Rich Extracts from Allium ursinum (Wild Garlic) Leaves: A Comparison of Conventional Maceration and Ultrasound-Assisted Extraction Techniques.

Despite the growing interest in using natural compounds for disease prevention and treatment, Allium ursinum (wild garlic), known for its therapeutic properties, has not been extensively studied for its chemical composition and biological activities. Therefore, this study aims to explore the in vitro antioxidant, antibacterial, and antitumor activities of A. ursinum extracts according to their functional phytochemical profile, while assessing whether ultrasound-assisted extraction (UAE) enhances bioactive properties in comparison to conventional maceration (CM). Both extracts were characterized by spectrophotometric methods and LC-ESI+-MS. The antioxidant activity was assessed via the CUPRAC and hydrogen peroxide scavenging assays, the antimicrobial properties via the disk-diffusion method against five pathogenic strains, and the antitumor activity via the MTT assay on four cancer cell lines. The major constituents of the methanolic extracts from leaves were kaempferol derivatives and alliin. The quercetin derivative rutin was also found. Maceration assisted using UAE yielded 20% more bioactive compounds in comparison to CM alone. Employing UAE in the extraction significantly increased antioxidant and antimicrobial proprieties, in line with its chemical composition. The antitumor cytotoxic activity was low to moderate, regardless of method, as explained by the absence of highly cytotoxic compounds. Wild garlic extracts possessed strong antioxidant and substantial antibacterial activities.