Exogenous auxin-induced ENHANCER OF SHOOT REGENERATION 2 (ESR2) enhances femaleness of cucumber via activating CsACS2 gene.

Cucumber (Cucumis sativus L.) is a model for the study of sex differentiation in the last two decades. In cucumber, sex differentiation is mainly controlled by genetic material, but plant growth regulators can also influence or even change it. However, the effect of exogenous auxin application on cucumber sex differentiation is mostly limited in physiological level. In this study, we explored the effects of different exogenous auxin concentrations on the varieties with different mutant sex-controlling genotypes and found that there was a dosage effect of exogenous indole-3-acetic acid (IAA) on the enhancement of cucumber femaleness. Several ACC synthetase (ACS) family members could directly respond to the induction of exogenous IAA to improve endogenous ethylene synthesis, and this process can be independent on the previously identified sex-related ACC oxidase CsACO2. We further demonstrated that ENHANCER OF SHOOT REGENERATION 2 (ESR2), responding to the induction of exogenous auxin, could directly activate CsACS2 expression by combining the ERE cis-acting element regions in the promoter, and then increase endogenous ethylene content, which may induce femaleness. These findings reveal that exogenous auxin improves cucumber femaleness via inducing sex-controlling gene and promoting ethylene synthesis.