Leptadenia hastata Leaf Extract ameliorates oxidative stress and serum biochemical parameters in Streptozotocin-Induced diabetes in Wistar rats.

INTRODUCTION: : Diabetes Mellitus is a major health problem characterized by hyperglycemia and disturbances in metabolism and implicated in causing oxidative stress. Treatment includes administration of oral hypoglycaemic agents with lifestyle modifications, these offer glycemic control, however, present limitations about availability, affordability and side effects. Traditional anti-diabetic plants are becoming popular in management of diabetes mellitus. This study was carried out to determine the efficacy of Leptadenaia hastata in treatment of diabetes. MATERIALS AND METHODS: Diabetes mellitus was induced in using a single injection of streptozotocin (50 mg kg(- 1) i.p.). The rats were divided into four groups of 5 rats each. Groups 3-6 received olive oil, 100 mg kg(- 1) extract, 200 mg.kg(- 1) extract and insulin (6IU kg(- 1)), respectively. 10 non-diabetic rats were grouped into two group receiving olive oil and 200 mg kg(- 1) extract for 28 days. All groups were sacrificed by injecting with ketamine hydrochloride, blood was collected by cardiac puncture and centrifuged. The serum was analyzed for biochemical parameters. The liver was removed and homogenized with the supernatant of the resultant homogenate collected and used for analysis of oxidative stress enzymes. RESULTS: The extract significantly decreased serum AST (p < 0.05), ALP (p < 0.001), ALT (p < 0.05), TG (p < 0.01), TC (p < 0.001), creatinine (p < 0.001). It had no effect on SOD and CAT levels but it significantly increased (p < 0.001) GSH levels and reduced (p < 0.05) MDA level. CONCLUSIONS: The n-hexane extract of Leptadenia hastata significantly decreased the levels of hepatic and renal serum biomarkers proving that it was beneficial in ameliorating diabetic related complications. The extract significantly increased GSH levels and reduced MDA level. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s40200-022-01017-z.