Chemotactic response of p-nitrophenol degrading Pseudomonas asiatica strain PNPG3 through phenotypic and genome sequence-based in silico studies.

The Pseudomonas asiatica strain PNPG3 was documented to possess chemotactic potential toward p-nitrophenol (PNP), and other nitroaromatic compounds. Initial screening with drop plate and swarm plate assays demonstrated significant movement of the strain toward the test compounds. A quantitative capillary assay revealed the highest chemotactic potential of the strain toward 4-Aminophenol (4AP), (CI: 12.33); followed by p-benzoquinone (PBQ), (CI: 6.8); and PNP, (CI: 5.33). Gene annotation revealed the presence of chemotactic genes (Che), (Methyl-accepting Proteins) MCPs, rotary motor proteins, and flagellar proteins within the genome of strain PNPG3. The chemotactic machinery of the strain PNPG3 comprised of thirteen Che genes, twenty-two MCPs, eight rotary motors, and thirty-four flagellar proteins that are involved in sensing chemoattractant. Two chemotactic gene clusters were recorded in the genome, of which the major cluster consisted of two copies of CheW, one copy of CheA, CheY, CheZ, one MotD gene, and several Fli genes. Various conserved regions and motifs were documented in them using a standard bioinformatics tool. Genes involved in the chemotaxis of strain PNPG3 were compared with three closely related strains and one distantly related strain belonging to Burkholderia sp. Considering these phenotypic and genotypic data, it can be speculated that it is metabolism-dependent chemotaxis; and that test compound activated the Che. This study indicated that strain PNPG3 could be used as a model organism for the study of the molecular mechanism of chemotaxis and bioremediation of PNP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s13205-023-03809-3.