Eco-Friendly Extraction and Formulation of Black Sea Shark Liver Oil-Based Emulgel for Anti-Inflammatory and Healing Dermatocosmetic Applications.

This study explores the eco-friendly extraction and formulation of emulgels based on Black Sea shark liver oil for their potential anti-inflammatory and wound healing dermatocosmetic applications. Two emulgel formulations were prepared: Gel 1, containing shark liver oil, and Gel 2, combining shark liver oil with borage oil. The eco-friendly extraction of shark liver oil was performed, yielding a high content of polyunsaturated omega-3 fatty acids, primarily eicosapentaenoic acid (16.68 ± 0.28 mg/g %) and docosahexaenoic acid (18.14 ± 0.31 mg/g %). Physicochemical evaluations of the emulgels revealed excellent stability over time, with small variations in pH, viscosity, and spreadability, confirming their robustness. Rheological analysis demonstrated pseudoplastic behavior for both formulations, with Gel 2 exhibiting a more favorable flow and consistency index (K = 34.11, n = 0.28) compared to Gel 1 (K = 32.73, n = 0.29). The anti-inflammatory effect was evaluated using two experimental edema models: 10% kaolin suspension and 6% dextran solution. Both emulgels demonstrated significant edema reduction, with Gel 2 showing a more potent anti-inflammatory effect. The wound healing effect was assessed in vivo, revealing that Gel 2 accelerated wound closure and hair restoration, outperforming Gel 1 and the reference diclofenac gel. These results suggest that Black Sea shark liver oil-based emulgels, especially borage oil formulation, offer promising eco-friendly alternatives for dermatocosmetic applications, with enhanced anti-inflammatory and wound healing properties.