A colorimetric and ratiometric fluorescent sensor for sequentially detecting Cu(2+) and arginine based on a coumarin-rhodamine B derivative and its application for bioimaging.

In this work, a colorimetric and ratiometric fluorescent sensor based on a coumarin-rhodamine B hybrid for the sequential recognition of Cu(2+) and arginine (Arg) via the FRET mechanism was designed and synthesized. With the addition of Cu(2+), the solution displayed a colorimetric change from pale yellow to pink which is discernible by the naked eye. Additionally, the fluorescence intensities of the sensor exhibited ratiometric changes for the detection of Cu(2+) at 490 and 615 nm under a single excitation wavelength of 350 nm, which corresponded to the emissions of coumarin and rhodamine B moieties, respectively. The fluorescence color change could be visualized from blue to pink. The limits of detection were determined to be as low as 0.50 and 0.47 μM for UV-vis and fluorescence measurements, respectively. More importantly, the sensor not only can recognize Cu(2+) and form a sensor-Cu(2+) complex but can also sequentially detect Arg with the resulting complex. The detection limits for Arg were as low as 0.60 μM (UV-vis measurement) and 0.33 μM (fluorescence measurement), respectively. A fluorescence imaging experiment in living cells demonstrated that the fabricated sensor could be utilized in ratiometric fluorescence imaging towards intracellular Cu(2+), which is promising for the detection of low-level Cu(2+) and Arg with potentially practical significance.