Polymeric Microparticles of Calcium Pectinate Containing Urea for Slow Release in Ruminant Diet.

In ruminant feeding, mechanisms for controlling the rate of ammonia release in the rumen are important for increasing the efficiency of transforming dietary nitrogen into microbial protein. Three microencapsulated formulations, with increased urea concentrations of 10 (MPec1), 20 (MPec2) and 30% (MPec3) from the w/w, based on the mass of citrus pectin solution, employ the external ionic gelation/extrusion technique. The properties of microencapsulated urea were examined as a completely randomized design with 5 treatments each with 10 replicates for evaluation, and the ratios of dietary to free urea were compared using 5 fistulated male Santa Ines sheep in a Latin 5 × 5 square design. The degradation kinetics showed that the rate of controlled release from the microencapsulated systems was significantly reduced compared with that of free urea (p < 0.05). The population density of ruminal protozoa increased when sheep received the microencapsulated urea (p < 0.05). The disappearance of dry matter and crude protein reached a degradation plateau during the first minutes for the MPec1 and MPec2 systems and was slower for MPec3. The MPec1 and MPec2 systems presented higher (p < 0.05) blood serum concentrations of albumin, urea nitrogen (BUN), creatinine and total cholesterol and did not affect (p > 0.05) the other blood metabolites. The MPec2 systems are recommended because they consist of microspheres with more (p < 0.05) controlled core release, delaying the peak of urea released in the rumen and BUN without affecting (p < 0.05) ruminal pH and temperature. Microencapsulation with calcium pectinate provided better utilization of urea, reducing the risk of ruminant intoxication.