LC3A-mediated autophagy regulates lung cancer cell plasticity

LC3A介导的自噬调节肺癌细胞可塑性

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作者:Chia-Cheng Miao, Wen Hwang, Ling-Yi Chu, Li-Hao Yang, Cam-Thu Ha, Pei-Yu Chen, Ming-Han Kuo, Sheng-Chieh Lin, Ya-Yu Yang, Shuang-En Chuang, Chia-Cherng Yu, Shien-Tung Pan, Mou-Chieh Kao, Chuang-Rung Chang, Yu-Ting Chou

Abstract

ATG14: autophagy related 14; CDH2: cadherin 2; ChIP-qPCR: chromatin immunoprecipitation quantitative polymerase chain reaction; CQ: chloroquine; ECAR: extracellular acidification rate; EMT: epithelial-mesenchymal transition; EPCAM: epithelial cell adhesion molecule; MAP1LC3A/LC3A: microtubule associated protein 1 light chain 3 alpha; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP1LC3C/LC3C: microtubule associated protein 1 light chain 3 gamma; NDUFV2: NADH:ubiquinone oxidoreductase core subunit V2; OCR: oxygen consumption rate; ROS: reactive oxygen species; RT-qPCR: reverse-transcriptase quantitative polymerase chain reaction; SC: scrambled control; shRNA: short hairpin RNA; SNAI2: snail family transcriptional repressor 2; SOX2: SRY-box transcription factor 2; SQSTM1/p62: sequestosome 1; TGFB/TGF-β: transforming growth factor beta; TOMM20: translocase of outer mitochondrial membrane 20; ZEB1: zinc finger E-box binding homeobox 1.

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