Evaluation of Different Concentrations of Antimicrobial Quaternary Polymers on the Behavior of Gelatin- and Starch-Based Films.

Nowadays, incorporating quaternary ammonium groups into polymers is one of the most promising strategies for preparing antimicrobial biomaterials for general applications. The main objective of this work was to evaluate the effect of different concentrations of antimicrobial quaternary polymers in gelatin- and starch-based films for the development of active materials intended for applications in food packaging and medical fields. Two antimicrobial biobased polymers, called MeFPIAx (MeFPIA1 and MeFPIA2), were previously synthesized through the radical polymerization of itaconic acid (IA), followed by their subsequent functionalization and modification. Both polymers were incorporated into a new blend of gelatin and starch (15% w/w, 4:1 mass ratio), using glycerol (30% w/w) as a plasticizer. Films were prepared using the casting technique from aqueous dispersions of the polymers and their structure was characterized by Fourier Transform Infrared Spectroscopy with Attenuated Total Reflectance (FTIR-ATR). The findings of this study showed the addition of MeFPIAx had a significant effect (p < 0.05), resulting in films with higher tensile strength (TS) and a higher Young's modulus (YM), with values close to 20 MPa and exceeding 250 MPa, respectively. On the other hand, elongation at break (EB) values lower than 80% were obtained. Additionally, the swelling was reduced from ~400% to 100% and a reduction in water vapor permeability (P(w)) was observed, thanks to the increased interaction between the polymeric chains. Differential scanning calorimetry (DSC) scans showed that the addition of MeFPIAx increased the glass transition temperatures (T(g)) from 29 °C to 65 °C. Furthermore, thermogravimetry analysis (TGA) indicated an increase in the initial degradation temperatures, suggesting that the films were more thermally resistant. Finally, the films exhibited slight antioxidant activity but significant antimicrobial activity, achieving bacterial reduction values greater than 70% with the incorporation of MeFPIAx polymers against Gram-positive Staphylococcus aureus.