Meeting Proceedings from 4th Minimal Cell Workshop: Exploring JCVI Minimal Cell Fundamental Insights and Integrative Applications.

The annual Minimal Cell Workshop, hosted by the J. Craig Venter Institute (JCVI), is an international virtual seminar that brings together over 80 academic, industrial, and government laboratories. Researchers use the JCVI's minimal bacterial cell platform to explore the principles of cellular life and integrate new chemical pathways. Since its creation in 2016, this platform has fostered global collaborations. The fourth workshop featured 26 talks on ongoing research with minimal cell strains, including JCVI-syn1.0, JCVI-syn3.0, JCVI-syn3A, and JCVI-syn3B. Topics included innovative imaging techniques like super-resolution microscopy and cryotomography, DNA replication assays, and minimal cell division. New approaches for ATP synthesis and the role of moonlighting proteins were also discussed. JCVI-syn3B applications explored its potential in understanding persistent pathogens and as an anticancer therapeutic. The workshop encouraged sharing techniques for cell culture and genetic manipulation, fostering collaboration and advancing efforts to develop genome-scale algorithms for understanding and manipulating cellular functions.