The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.
Development of a universal glycosyltransferase assay amenable to high-throughput formats.
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作者：Lee Hyun Soo, Thorson Jon S
| 期刊： | Analytical Biochemistry | 影响因子： | 2.500 |
| 时间： | 2011 | 起止号： | 2011 Nov 1; 418(1):85-8 |
| doi： | 10.1016/j.ab.2011.06.016 | ||
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