Human Cytomegalovirus UL78 is a Nuclear-Localized GPCR Necessary for Efficient Reactivation from Latent Infection in CD34(+) Hematopoietic Progenitor Cells.

Human cytomegalovirus (HCMV) is a ubiquitous pathogen that persists throughout the lifetime of the host due in part to the establishment of latency in CD34(+) hematopoietic progenitor cells (HPCs) and CD14(+) monocytes. HCMV encodes four putative G protein-coupled receptors (GPCRs): US27, US28, UL33, and UL78. While the roles of most of these receptors have been investigated, a definitive role for UL78 in HCMV infection has yet to be elucidated. Utilizing an in vitro CD34(+) HPC model, we demonstrate that a recombinant virus lacking UL78 protein expression fails to efficiently reactivate from latent infection. Furthermore, we show using a Lumit-based assay that UL78 preferentially couples to the Gα(i) family of G proteins and that a recombinant HCMV containing mutations in the UL78 G protein-coupling DRL motif also fails to reactivate from latent infection. Together our findings indicate that Gα(i) coupling is important for UL78 function during reactivation in latently infected CD34(+) HPCs, however the protein is not required to establish or maintain latency. To better understand the role of UL78, we conducted proximity-dependent labeling analyses in HCMV-UL78-TurboID infected fibroblasts and CD34(+) HPCs undergoing reactivation from latency. Congruent with our coupling data, we found Gα(i) was the only heterotrimeric Gα protein in proximity to UL78. Pathway analysis of the UL78 interactome revealed proteins associated with membrane trafficking, signaling, and the nuclear pore complex as enriched in both cell types. In addition, the UL78 interactome contained viral proteins with nuclear localization including viral transcription and DNA replication machinery. Nuclear localization of UL78 was validated using cell fractionation, immunofluorescence microscopy, and proximity-dependent labelling of isolated nuclei. Together, our results provide novel insights into the localization and function of UL78, previously unknown to contribute to reactivation from latent infection.