Immobilization of C. elegans with different concentrations of an anesthetic for time-lapse imaging of dynamic protein trafficking in neurons.

Here we compare the percentage of anterograde and retrograde trafficking events as well as the average velocity of these events in worms immobilized with microbeads or 0.5-7.5 mM tetramisole. Our results show that the percentage and average velocity of TIR-1 ::GFP moving events in the C. elegans AWC axons are not significantly different between worms immobilized with 7.5 mM tetramisole and other conditions. Our results suggest that 7.5 mM tetramisole, compared to 0.5 mM, 1 mM, and 2 mM tetramisole, does not have a significant effect on the axonal transport of TIR-1 ::GFP along the AWC axons.