Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and is compatible with super-resolution microscopy. The combination of these properties makes this probe a powerful tool for live-cell imaging.
SiR-Hoechst is a far-red DNA stain for live-cell nanoscopy.
SiR-Hoechst 是一种用于活细胞纳米显微镜的远红外 DNA 染料
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作者：LukinaviÄius Gražvydas, Blaukopf Claudia, Pershagen Elias, Schena Alberto, Reymond Luc, Derivery Emmanuel, Gonzalez-Gaitan Marcos, D'Este Elisa, Hell Stefan W, Wolfram Gerlich Daniel, Johnsson Kai
| 期刊： | Nature Communications | 影响因子： | 15.700 |
| 时间： | 2015 | 起止号： | 2015 Oct 1; 6:8497 |
| doi： | 10.1038/ncomms9497 | 研究方向： | 细胞生物学 |
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