α-Synuclein Sequences from Long-Lived Animals Display Generally Diminished Aggregation Compared to Shorter-Lived Animals Including Humans.

The overall process of protein aggregation from soluble species to amyloid fibrils is toxic to neurons and can propagate along neuronal connections in ways that potentially explain the pathological progression in most neurodegenerative diseases. One of these aggregation-prone proteins is α-synuclein (α-Syn), which forms insoluble protein deposits in Parkinson's disease and other synucleinopathies. The majority of cases of Parkinson's disease occur fairly late in life, and even early-onset variants of the disease caused by mutations to α-Syn occur toward the end of the lifespan for prehistoric man. This suggests a lack of evolutionary pressure to prevent protein aggregation in animals with similar or shorter lifespans. However, α-Syn is also found in animals with notably longer lifespans. Here, this study tests the aggregation propensity of α-Syn sequences from short- and longer-lived animals at a range of evolutionary distances from humans. This study finds that, in general, longer-lived animals display slower α-Syn aggregation kinetics and the formation of smaller and less uniform fibrils. Overall, data indicate that some evolutionary pressure may have existed for preventing α-Syn aggregation, but that pressure is lost in the more recent branch of shorter-lived animals containing humans.