Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa

顺磁性纤维素 DNA 分离可提高不同植物类群的 DNA 产量和质量

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作者:Jackson R Moeller, Nicholas R Moehn, Donald M Waller, Thomas J Givnish

Conclusions

PMC technology is a promising alternative for plant DNA isolation.

Results

Yield and purity of DNA isolated by PMC, DNeasy Plant Mini Kit (silica column), and cetyltrimethylammonium bromide (CTAB) methods were compared among four individuals for each of 25 plant species. PMC gave a twofold advantage in average yield, and the relative advantage of the PMC method was greatest for samples with the lowest DNA yields. PMC also produced more consistent sample purity based on absorbance ratios at 260:280 and 260:230 nm. • Conclusions: PMC technology is a promising alternative for plant DNA isolation.

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