Microsatellite primer development for post oak, Quercus stellata (Fagaceae)

星栎(壳斗科)微卫星引物开发

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作者:Warren B Chatwin, Kyrie K Carpenter, Felix R Jimenez, Dave B Elzinga, Leigh A Johnson, Peter J Maughan

Conclusions

We report the development of microsatellite markers, specific to post oak, to aid the study of genetic diversity and population structure of extant forest remnants.

Results

Two Q. stellata individuals, sampled from the northern and southern range of the post oak forest, were subject to genomic reduction and 454 pyrosequencing. Bioinformatic analysis identified putative microsatellites from which 12 polymorphic primer sets were screened on three populations. The number of alleles observed ranged from five to 20 across all populations, while observed and expected heterozygosity values ranged from 0.05 to 0.833 and 0.236 to 0.893, respectively, within individual populations. • Conclusions: We report the development of microsatellite markers, specific to post oak, to aid the study of genetic diversity and population structure of extant forest remnants.

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