Integrative metabolomics and molecular networking reveal metabolic rewiring in Tartary buckwheat sprouts under moderate hydrostatic pressure.

The effects of moderate hydrostatic pressure (MHP) pretreatment on bioactive metabolites in Tartary buckwheat sprouts remain insufficiently studied. In this work, a non-targeted metabolomics approach, combined with feature-based molecular networking (FBMN), identified 22 metabolites in sprouts treated with 0-30 MPa pressure, four of which were previously unreported, thus expanding the species' phytochemical diversity. MHP treatment activated phenylalanine ammonia-lyase (PAL), resulting in a 2.3-fold increase in total flavonoids, particularly glycosylated derivatives such as quercetin 3-rutinoside 7-glucoside (13.2-fold increase at 25 MPa). In contrast, condensed tannins, responsible for astringency, were reduced by 40-60 %. These findings suggest that MHP pretreatment may serve as a sustainable alternative to chemical elicitors, promoting biofortification and improving sensory attributes in sprout-based functional foods.