Leukotriene D(4) induces chemotaxis in human eosinophilc cell line, EoL-1 cells via CysLT1 receptor activation.

Numerous reports have shown that cysteinyl leukotrienes (CysLTs) contribute to tissue accumulation of eosinophils in allergic airway inflammation. To date, only a few studies have reported that CysLTs promote chemotactic activity of human eosinophils in vitro. The purpose of this study was to investigate whether CysLTs promote chemotaxis in the human eosinophilic cell line, EoL-1. EoL-1 cells were induced to differentiate into mature eosinophil-like cells via incubation with butyric acid and cytokines (IL-3, IL-5 and GM-CSF). The chemotactic activity of the differentiated EoL-1 cells was assessed using the commercial cell migration assay kit. LTD(4) elicited dose-related chemotactic activity in the differntiated EoL-1 cells in the range of 1-100 nM. A typical bell-shaped dose-response curve was observed with optimal activity at 10 nM. The chemotactic activity elicited by LTD(4) (10 nM) was significantly inhibited by montelukast (control, 345 ± 19.2 × 10(3) RFU; LTD(4) 10 nM alone, 511 ± 39.2 × 10(3) RFU; LTD(4) 10 nM plus montelukast 100 nM, 387 ± 28.2 × 10(3) RFU). LTD(4) induces migration in eosinophilic cells via activation of CysLT1 receptor. The present in vitro model may be useful for elucidation of the mechanism underlying CysLT-induced tissue eosinophilia.