Genomic Insight into Shimazuella Soli Sp. Nov. Isolated from Soil and Its Putative Novel Class II Lasso Peptide.

The strain designated as AN120528(T) was isolated from farmland soil in South Korea. This strain grows well on R2A medium at 28 °C. The cells are an off-white colour and have no hyphae. The phylogenetic analysis indicated that the strain is a member of the genus Shimazuella with a 98.11% similarity to Shimazuella alba KC615(T) and a 97.05% similarity to S. kribbensis KCTC 9933(T), respectively. The strain AN120528(T) shares common chemotaxonomic features with the other two type strains in the genus. It has MK-9 (H(4)) and MK-10 (H(4)) as its predominant menaquinones. The major fatty acids are iso-C(14:0), iso-C(15:0), anteiso-C(15:0) and iso-C(16:0). Diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), lipids (L), and aminolipids (AL) were identified as the major cellular polar lipids. Analysis of the peptidoglycan showed the presence of meso-diaminopimelic acid. Whole-genome sequencing revealed that the genome of the strain is approximately 3.3 Mbp in size. The strain showed a 77.5% average nucleotide identity (ANI) with S. alba KC615(T). The genomic DNA (gDNA) G + C content is 39.0%. Based on polyphasic taxonomy analysis, it is proposed that this strain, AN120528(T), represents a novel species in the genus Shimazuella, designated as Shimazuella soli sp. nov. The type stain is AN120528(T) (=KCTC 39810(T) = DSM 103571(T)). Furthermore, shimazuellin I, a new 15-amino-acid peptide, was discovered in the AN120528(T) through genome mining; it has the features of a lasso peptide, containing eight amino acids (-G-Q-G-G-S-N-N-D-) that form a macrolactam ring and seven amino acids (-D-G-W-Y-H-S-K-) that form a tail.