Conclusion
The beneficial effects of BRP could be used as an immunotherapeutic adjuvant in treatment of inflammatory diseases.
Methods
The crude polysaccharide from roots of B. rapa (BRP) was extracted and purified to further investigate the active fraction of BRT for inducing macrophage phagocytosis.
Objective
To investigate the immunomodulatory activity of polysaccharides from the roots of Brassica rapa.
Results
Effects on RAW264.7 cells demonstrated that BRP behaved better phagocytic capacity and had potent immunomodulatory activity, including increasing production of nitric oxide (NO), tumor necrosis factor α (TNFα) and upregulating mRNA levels of inducible NO synthase (iNOS) and TNFα. Furthermore, modulation of macrophage by BRP was indicated to be mediated via the activation of Akt and nuclear factor-kappa B (NF-κB).