M13KO7 bacteriophage enables Potato Virus Y detection

M13KO7噬菌体可检测马铃薯Y病毒

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作者:Haneul Seo #, Sang-Ho Cho #, Thuy T B Vo #, Ahlim Lee, Sungrae Cho, Sol Kang, Eui-Joon Kil, Hee-Seong Byun, Mi-Gi Lee, Myung-Hee Kwon, Woo-Jae Chung, Young-Gyu Lee, Sukchan Lee

Abstract

In this study, we confirmed the binding of M13KO7 to Potato virus Y (PVY) using enzyme-linked immunosorbent assay. M13KO7 is a "bald" bacteriophage in which no recombinant antibody is displayed. M13KO7 is easy to propagate by using Escherichia coli, making this method more reasonable in economic perspective. Based on this study, we suggest that M13KO7 detection system has applicability as a novel biological tool for the detection of PVY.

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