CK beta 8/CCL23 induces cell migration via the Gi/Go protein/PLC/PKC delta/NF-kappa B and is involved in inflammatory responses

CKbeta8/CCL23 is a CC chemokine and alternative splicing of the CKbeta8 gene produces two mRNAs that encode CKbeta8 and its isoform CKbeta8-1. Although it has been reported that CKbeta8 and CKbeta8-1 are implicated in leukocyte trafficking and development of inflammation, the exact roles of these two chemokines in immune responses and the associated chemotaxis signaling are still obscure. Main

To understand the mechanism of CKbeta8- and CKbeta8-1-induced chemotaxis signaling, we examined the chemotactic activities of osteogenic sarcoma cells expressing CC chemokine receptor 1 in response to CKbeta8 and CKbeta8-1. We also examined involvement of CKbeta8 and CKbeta8-1 in inflammatory responses by determining the mRNA expression of pro-inflammatory molecules induced by two chemokines and expressions of these chemokines in foam cells. Key findings:

These results indicate that both CKbeta8 and CKbeta8-1 transduce the chemotaxis signal through the G(i)/G(o) protein, PLC, PKCdelta, and NF-kappaB, and that CKbeta8 and CKbeta8-1 probably play important roles in inflammatory diseases such as atherosclerosis.