Analysis of significance of CARD11 and MYO1G expressions in pulmonary tuberculosis and their predictive value for prognosis of recurrence

in English, Serbian Pulmonary tuberculosis (PTB) is one of the most common infectious diseases worldwide, with extremely high morbidity and mortality. An in-depth understanding of the molecular pathogenesis of PTB is crucial for finding novel diagnostic and therapeutic approaches in the future. In this study, we identified 52 differentially expressed genes (DEGs) from the GSE34608 dataset in the Gene Expression Omnibus (GEO) database, and the protein-protein interaction (PPI) network showed that these DEGs included 42 nodes and 20 edges. We found that CARD11 and MYO1G were closely associated with T cell activation by functional enrichment analysis. Upon further clinical case analysis, we found that the expressions of CARD11 and MYO1G in the peripheral blood of patients with PTB were lower than those of healthy individuals. Meanwhile, through Receiver operating characteristic (ROC) curve analysis, we found that CARD11 and MYO1G showed excellent diagnostic effects on the occurrence and prognosis of PTB recurrence. Thus, CARD11 and MYO1G are promising indicators for assessing PTB in the future. Tuberkuloza pluća (PTB) je jedna od najčešćih zaraznih bolesti u svetu, sa izuzetno visokim stopama morbiditeta i mortaliteta. Dublje razumevanje molekularne patogeneze PTB je ključno za pronalaženje novih dijagnostičkih i terapijskih pristupa u budućnosti. U ovoj studiji smo identifikovali 52 različito eksprimirana gena (DEG) iz skupa podataka GSE34608 u bazi Gene Expression Omnibus (GEO), a mreža interakcija protein-protein (PPI) je pokazala da ovi DEG uključuju 42 čvora i 20 ivica. Utvrdili smo da su CARD11 i MYO1G blisko povezani sa aktivacijom T-ćelija putem analize funkcionalnog obogaćivanja. Daljom analizom kliničkih slučajeva, utvrdili smo da su ekspresije CARD11 i MYO1G u perifernoj krvi pacijenata sa PTB bile niže u odnosu na zdrave pojedince. Takođe, analizom ROC krive smo utvrdili da CARD11 i MYO1G pokazuju izuzetne dijagnostičke efekte u pogledu pojave i prognoze recidiva PTB. Stoga su CARD11 i MYO1G indikatori za procenu PTB koji obećavaju. Keywords: CARD11; MYO1G; T cell activation; analysis of biological information; pulmonary tuberculosis.