Abstract
The cellular secretory pathway is a fundamental physiological process; yet a precise, efficient, and quantitative method for monitoring this process remains seldom explored. In this study, we achieved real-time monitoring of the secretion of angiotensin-converting enzyme 2 and programmed death-ligand 1 by integrating the HiBiT tag with the classic Retention Using Selective Hooks system. Through this strategy, we identified and validated Niemann-Pick complex 1 as a lysosomal adaptor involved in the angiotensin-converting enzyme 2 trafficking process. We propose that the strategy offers a valuable tool for the precise and rapid identification of diverse trafficking regulators and therapeutic compounds targeting various receptors or cytokines, thereby advancing the field of cellular transport research.