Mastery of cultural conditions and physico-chemical properties improves the production and the catalytic efficiency of bglG.

Stachybotrys microspora is a filamentous fungus secreting multiple β-glucosidases. Two of them were characterized. The third one, named bglG, was also characterized and used for various investigations. The current work undertakes the plausible role played by some cultural conditions and physico-chemical properties to improve bglG time course synthesis and also its catalytic efficiency. Indeed, bglG time course synthesis is slightly affected by light, but it is clearly affected by aeration and presence of baffle. On the same case, optimization of substrate and enzyme concentration contributes to the improvement of the catalytic efficiency of bglG. This biocatalyst tolerates a high ionic strength during its activity assay; β-mercaptoethanol increases the enzymatic rate. BglG has the capacity to hydrolyse efficiently oleuropéin, with a recovery of 88%.