Microaggregates as Nutrient Reservoirs for Fungi Drive Natural Regeneration in Larch Plantation Forests.

The natural regeneration of Larix gmelinii plantations plays a pivotal role in rehabilitating ecosystem services in Northeast China's degraded forests. However, mechanistic linkages between soil aggregate nutrient fluxes and fungal community assembly remain poorly constrained. Combining space-for-time substitution with particle-size fractionation and high-throughput sequencing, this study examined successional trajectories across regeneration in Langxiang National Nature Reserve to resolve nutrient-fungal interplay during long-term forest restructuring. The results demonstrated that microaggregates (<0.25 mm) functioned as nutrient protection reservoirs, exhibiting significantly higher total carbon (TC) and nitrogen (TN) contents and greater fungal diversity (p < 0.05). Both stand regeneration stage and aggregate size significantly influenced fungal community composition and structural organization (p < 0.05). Aggregate-mediated effects predominated in upper soil horizons, where fungal dominance progressively transitioned from Mortierellomycota to Ascomycota with increasing particle size. In contrast, lower soil layers exhibited regeneration-dependent dynamics: Basidiomycota abundance declined with L. gmelinii reduction, followed by partial recovery through mycorrhizal reestablishment in Pinus koraiensis broadleaf communities. Fungal co-occurrence networks displayed peak complexity during Juglans mandshurica germination (Node 50, Edge 345), with 64.6%positive correlations, indicating the critical period for functional synergy. Basidiomycota showed significant negative correlations with nutrients and major fungal phyla (R(2) = 0.89). This study confirms that natural vegetation regeneration reshapes belowground processes through litter inputs and mycorrhizal symbiosis, while microaggregate management enhances soil carbon sequestration. Near-natural plantation management should incorporate broadleaf species to preserve mycorrhizal diversity and amplify ecosystem services. These findings provide an essential soil ecological theoretical basis for sustainable plantation management in Northeast China.