Pretreatment of Japanese cedar by ionic liquid solutions in combination with acid and metal ion and its application to high solid loading

Lignocellulosic biomass from plant biomass, especially softwoods, are well-known to present difficulties during attempts at hydrolysis due to their rigid structure. Pretreatment of lignocellulosic biomass with ionic liquids (ILs) is attractive as this requires to a low input of energy. However, IL pretreatment has the disadvantage of the presence of large amounts of water. Recently, it was reported that a small amount of acid has a positive effect on the degradation of biomass in IL with water. In this study the pretreatment of Japanese cedar, the most abundant softwood in Japan, was investigated using a combination of IL, acid and metal ions.

We developed an effective pretreatment protocol by changing to a pretreatment solvent containing IL, acid, metal ion and anti-solvent. The optimized pretreatment has an effect on softwood and separately retrieved lignin as a by-product. The saccharified solution at high-solid biomass loading was converted to ethanol in a high yield. This proposed methodology would boost the performance of the bioconversion of low-cost materials to other chemicals, and would not be limited to only ethanol but also would include other target chemicals.

First, the novel ionic liquid pretreatment was investigated by changing the pretreatment solvent and the anti-solvent. A mixture of IL, acid and ferric oxide (Fe(3+)) ion was most effective for pretreatment, and an acetone-water mixture was also most effective on the precipitation of biomass. These optimized pretreatment combinations attained a higher degree of glucose release from the pretreated biomass. The amount of cellulose was concentrated from to a level of 36 to 84% of the insoluble fraction by the optimized pretreatment. Based on this result, it was assumed that the extraction of the lignin fraction from the biomass into an anti-solvent solution was attained. Finally, this optimized pretreatment was applied to the enzymatic hydrolysis of Japanese cedar at high-solid biomass loading, and 110 g/L of glucose production was attained. In addition, the ethanol fermentation with this hydrolyzed solution by Saccharomyces cerevisiae achieved 50 g/L ethanol production, and this yield reached 90% of the theoretical yield. Conclusions: We developed an effective pretreatment protocol by changing to a pretreatment solvent containing IL, acid, metal ion and anti-solvent. The optimized pretreatment has an effect on softwood and separately retrieved lignin as a by-product. The saccharified solution at high-solid biomass loading was converted to ethanol in a high yield. This proposed methodology would boost the performance of the bioconversion of low-cost materials to other chemicals, and would not be limited to only ethanol but also would include other target chemicals.